Thursday, February 23, 2012
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Polyclonal Antibody Development Services
We offer polyclonal antibody development in mice, rabbits, chickens and goats.We can use proteins, peptides, nucleotides, and small molecules as antigens. We guarantee all our services. The table below gives an outlines of our typical services. Please review the information below and contact us for a specific quote to meet your needs.
| Item | Description | Time | Cost |
| ARC201 | Fast 4 week Mouse polyclonal development, 10mL Ascities | 4-5 weeks | $795 |
| ARC202 | Rabbit Polyclonal with Your Immunogen | 8-10 weeks | $595 |
| ARC203 | Rabbit Polyclonal with Peptide Synthesis & Conjugation | 10-12 weeks | $1,195 |
| ARC204 | Rabbit Polyclonal with Peptide Synthesis & Affinity Purification | 10-12 weeks | $1,495 |
| ARC205 | Chicken Polyclonal with Your Immunogen | 8-10 weeks | $495 |
| ARC206 | Chicken Polyclonal with Peptide Synthesis & Conjugation | 10-12 weeks | $1,095 |
| ARC207 | Chicken Polyclonal with Peptide Synthesis & Affinity Purification | 10-12 weeks | $1,495 |
| ARC208 | Goat Polyclonal Antibody Development | 10-12 weeks | Call |
| ARC209 | Polyclonal Development, Other species | 10-12 weeks | Call |
| ARC210 | Mouse Genetic (DNA) Immunization, No Protein / Peptide Needed | 5-6 weeks | Call |
Our 4-week short protocol is a hit with many clients. You pay only if we get a titer.
Our Polyclonal Antibody Development program is divided into the following phases.
These phases are optional and client can choose to decide what they want to do.
We work with our customers to decide the best approach to meet their requirement.
| Item | Description | Time |
| 1. | Phase-1 - Initiation & preparation of immunogen. Sequence analysis and validation. Peptide synthesis and conjugation to KLH or BSA. Preparation of DNA expression vector. Design and synthesis of small molecule immunogen with carrier. |
3-4 weeks |
| 2. | Phase-2 Immunization of animals (2 Rabbits, 2 Chicken, 2 mice or 2 Goat) with antigen and determination of antibody titer by ELISA. | 8-10 weeks |
| 3. | Phase-3 Purification of antibody. a) Immunogen affinity purification (antigen specific antibody IgG + IgM) b) Protein-A/G purification (Total IgG) |
1 week |
Choice of animal should depend on cost and amount of antibody needed (See table)
| Animal | Minimum Amount of Antigen Needed | Volume of Hyper Immune Sera | Amount of Purified Antibody |
| Mouse | 0.5mg | 10mL (ascites) | ~1 mg |
| Rabbit | 2.0mg | 100mL | ~10 mg |
| Chicken | 2.0mg | 100mL | ~10 mg |
| Goat | 10mg | 1000mL | ~100 mg |
